Primary structure of a high M r form of rat atrial natriuretic factor

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Structure and physiological actions of rat atrial natriuretic factor.

Natriuretic substances were purified from rat atrium (atrial natriuretic factor, ANF) and were shown to be identical with the inhibitor of norepinephrine-induced contraction of smooth muscle. Four native forms were isolated and their amino acid sequences were determined. The presence of a high-molecular-weight prohormone was shown. Complementary DNA (cDNA) encoding for the precursor was cloned ...

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Renal mechanism of action of rat atrial natriuretic factor.

There has been conflict as to whether crude extracts of atrial natriuretic factor increase renal solute excretion by a hemodynamic mechanism or by direct inhibition of tubular transport. To investigate this issue, seven rats were studied during a euvolemic control period and following continuous administration of pure, synthetic 24 amino acid atrial natriuretic factor. A 10-25-fold increase in ...

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Degradation of atrial natriuretic factor in the rat.

The biologically active circulating form of atrial natriuretic factor (ANF) in the rat is the 28-amino-acid peptide ANF-(Ser-99-Tyr-126). Degradation of this peptide in vivo as well as in vitro, in whole blood, in plasma and by the isolated mesenteric artery was investigated. Studies in vivo in the rat demonstrated that the elimination and degradation of ANF was extremely fast: within 3 min mor...

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Proximal regulatory domains of rat atrial natriuretic factor gene.

BACKGROUND At least three cis-acting regulatory elements are required for expression of the rat atrial natriuretic factor (ANF) gene. One distal cis-acting regulatory element lies more than 640 base pairs from the transcription initiation site. METHODS AND RESULTS In this report, we identify two other proximal regulatory elements that lie within 609 base pairs of the transcription initiation ...

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Effect of Extracellular Calcium Depletion on Atrial Natriuretic Factor Secretion and Gene Expression

     Extracellular Ca 2+ <span style="color: #231f20; font-family: Times ...

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ژورنال

عنوان ژورنال: FEBS Letters

سال: 1984

ISSN: 0014-5793

DOI: 10.1016/0014-5793(84)80155-6